Cultivation of <i>Gardenia jasminoides</i> shoots in temporary immersion systems and optimization of the immersion periods

In the present study, effect of different immersion regimes on growth and secondary metabolites production by shoot cultures Gardenia jasminoides Ellis, cultivated in temporary systems (TIS) was investigated. The cultivation performed for 30 days MS medium supplemented with 4.0 mg/L BAP. included 15 min. every 4, 8 12 hours. highest amount accumulated dry biomass (ADB = 10.86 ± 0.02 g/L) reached at hours, whereas lowest 4.65 0.26 recorded when 4 hours used. HPLC analyses showed that higher polyphenol content achieved shoots were using At this conditions, produced maximal amounts chlorogenic acid (164.82±32.36ug/g DW), caffeic (26,90±6,80μg/g rutin (891,67±122,29μg/g DW) rosmarinic (559,19±19,85μg/g DW). observed antioxidant activity (DPPH, ABTS, CUPRAC FRAP) following accumulation total phenolic. study demonstrates TIS are effective shoots.